Amuda Mutiu Olasunkanmi
Natural products from medicinal plants provide unlimited opportunities for new drugs leads. This study identify the polyphenols present in methanol fraction of persea americana stem bark by ultra-high performance liquid chromatography coupled with tandem mass spectrometer (LC-MS/MS), the Polyphenols were extracted using EtOH/H2O (80:20 v/v) and the extract obtained was subjected to successive maceration using three different solvents leading to hexane, ethyl-acetate, and methanol fraction which were labeled as PAHF, PAEF and PAMF respectively. Four polyphenols were identified in PEAF by LC-MS/MS analysis: Silybin B, Delphinidin, and Hersperetin. The major peaks observed from isolated fragmentation of silybin B were 481, 391, 167, and 149 m/z which were assigned to (M-H)-, (M-+C2H2O4), (+C8H8O4), (+C6H4O3) fragmented ions respectively. The total phenolic and flavonoid contents was determined. Phytochemical screening of the crude extract was also carried out.