Journal of Clinical Immunology Research
- ISSN: 2576-0572
Ihsan Edan Alsaimary, Ali Osama Ali, Ahmed Saad Abdulkhader and Husain Lateef Abd Hashosh
Infertility remains a global health challenge with devastating psycho-social consequences in many communities, and the underlying long-term risk of couple separation is also a major clinical and social problem. Infertility is defined as the inability of a couple to conceive naturally after one year of intercourse. The aim of the study was to determine the immune molecular characterization of TLR genes. Toll-like receptors (TLRs) are an important family of receptors that constitute the first line of defense against pathogens. They can recognize both invading pathogens and endogenous danger molecules released from dying cells and damaged tissues and play a key role in linking innate and adaptive immunity. TLRs are widely distributed in both immune and other body cells.
A cross sectional case control study was carried out by ELISA technique, conventional PCR, and DNA sequencing among male infertility patients who attended to the infertility and in vitro fertilization center of Basrah province on September 1st, 2021, and June 1st, 2022. A questionnaire paper was used to record special notes. Samples were collected from (176 patients and controls divided as 88 patients of male samples and 88 samples of the control group) including seminal fluid and blood. The molecular study in conventional polymerase chain reaction showed a clear and well-focused DNA band, and this indicates the presence of TLR genes in a high concentration, and the percentage of the detection was 100% for patients and control groups.